Coding

Part:BBa_K584021:Design

Designed by: Bakul Jitendra Vinchhi, Sarah Veugelen, Katrien Vandermeeren, Yana Hoorne   Group: iGEM11_KULeuven   (2011-09-17)

Ice Nucleating Protein Gene - inaZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 646
    Illegal NgoMIV site found at 2158
    Illegal NgoMIV site found at 2302
    Illegal NgoMIV site found at 2566
    Illegal NgoMIV site found at 2710
    Illegal NgoMIV site found at 2758
    Illegal NgoMIV site found at 2878
    Illegal AgeI site found at 417
    Illegal AgeI site found at 1822
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 592
    Illegal BsaI.rc site found at 3338
    Illegal SapI.rc site found at 238


Design Notes

Source

References

[1] Bassi AS Et al., Expression of single chain antibodies (ScFvs) for c-myc oncoprotein in recombinant Escherichia coli membranes by using the ice-nucleation protein of Pseudomonas syringae. Biotechnol Prog. 2000 Jul-Aug;16(4):557-63.